Analysis Note

Positive ControlHeLa cells, NIH3T3 cells, Human testes tissue

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Paraffin Sections (0.2 g/ml)

General description

This Anti-SALL4 Mouse mAb (6E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of SALL4.

Recognizes the ~100 kDa SALL4 protein in HeLa and NIH3T3 cells and human testes tissue.

Purified mouse monoclonal antibody. Recognizes the ~100 kDa SALL4 protein.

Immunogen

A recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein

NA

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cauffman, G., et al. 2009. Hum. Reprod.24 63.Forte, A., et al. 2009. Mol. Med.15 392.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µg in Glass bottle

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)